Cell‐cell signaling by direct contact increases cell proliferation via a PI3K‐dependent signal

FEBS Letters

Nelson, Celeste M¹  Chen, Christopher S¹ 

[1] Department of Biomedical Engineering, Johns Hopkins School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA

关键词: Cell shape;    Microcontact printing;    Intercellular adhesion;    Extracellular matrix;   

DOI  :  10.1016/S0014-5793(02)02370-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we found that introducing cell-cell contact positively regulates proliferation, but that contact-mediated proliferation can be masked by changes in cell spreading: Round cells with many contacts proliferated less than spread cells with none. Physically blocking cell-cell contact or inhibiting PI3K signaling abrogated cell-cell induced proliferation, but inhibiting diffusible paracrine signaling did not. Thus, direct cell-cell contact induces proliferation in these cells.

【 授权许可】

Unknown