FEBS Letters | |
Cell‐cell signaling by direct contact increases cell proliferation via a PI3K‐dependent signal | |
Nelson, Celeste M1  Chen, Christopher S1  | |
[1] Department of Biomedical Engineering, Johns Hopkins School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA | |
关键词: Cell shape; Microcontact printing; Intercellular adhesion; Extracellular matrix; | |
DOI : 10.1016/S0014-5793(02)02370-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we found that introducing cell-cell contact positively regulates proliferation, but that contact-mediated proliferation can be masked by changes in cell spreading: Round cells with many contacts proliferated less than spread cells with none. Physically blocking cell-cell contact or inhibiting PI3K signaling abrogated cell-cell induced proliferation, but inhibiting diffusible paracrine signaling did not. Thus, direct cell-cell contact induces proliferation in these cells.
【 授权许可】
Unknown
【 预 览 】
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RO201912020311575ZK.pdf | 323KB | download |