CYP98A6 from Lithospermum erythrorhizon encodes 4‐coumaroyl‐4′‐hydroxyphenyllactic acid 3‐hydroxylase involved in rosmarinic acid biosynthesis 1

FEBS Letters

Nagatsu, Akito²  Matsuno, Michiyo²  Ellis, Brian E¹  Mizukami, Hajime²  Ogihara, Yukio² 

[1] The Biotechnology Laboratory and Faculty of Agricultural Sciences, University of British Columbia, 6270 University Blvd., Vancouver, BC, Canada V6T 1Z3;Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467-8603, Japan

关键词: Cytochrome P450;    Rosmarinic acid biosynthesis;    3-Hydroxylation of phenolic ester;    Lithospermum erythrorhizon;    RA;    rosmarinic acid;    CHPL;    4-coumaroyl-4′-hydroxyphenyllactic acid;    PAL;    phenylalanine ammonia-lyase;    HPR;    4-hydroxyphenylpyruvate reductase;    C4H;    cinnamic acid 4-hydroxylase;    YE;    yeast extract;    MJ;    methyl jasmonate;   

DOI  :  10.1016/S0014-5793(02)02368-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rosmarinic acid is the dominant hydroxycinnamic acid ester accumulated in Boraginaceae and Lamiaceae plants. A cytochrome P450 cDNA was isolated by differential display from cultured cells of Lithospermum erythrorhizon, and the gene product was designated CYP98A6 based on the deduced amino acid sequence. After expression in yeast, the P450 was shown to catalyze the 3-hydroxylation of 4-coumaroyl-4′-hydroxyphenyllactic acid, one of the final two steps leading to rosmarinic acid. The expression level of CYP98A6 is dramatically increased by addition of yeast extract or methyl jasmonate to L. erythrorhizon cells, and its expression pattern reflected the elicitor-induced change in rosmarinic acid production, indicating that CYP98A6 plays an important role in regulation of rosmarinic acid biosynthesis.

【 授权许可】

Unknown