Noladin ether, a putative novel endocannabinoid: inactivation mechanisms and a sensitive method for its quantification in rat tissues

FEBS Letters

Di Marzo, Vincenzo²  Minassi, Alberto¹  Appendino, Giovanni¹  Bisogno, Tiziana²  Mechoulam, Raphael³  Fezza, Filomena² 

[1] DiSCAFF, Università del Piemonte Orientale, Viale Ferrucci 33, 28100 Novara, Italy;Endocannabinoid Research Group, Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, Comprensorio Olivetti, Fabbricato 70, 80078 Pozzuoli (Naples), Italy;Department of Medicinal Chemistry and Natural Products, Faculty of Medicine, Hebrew University, Jerusalem 91120, Israel

关键词: Anandamide;    2-Arachidonoyl glycerol;    2-Arachidonyl glyceryl ether;    Cannabinoid;    Endocannabinoid;    AEA;    anandamide;    AA;    arachidonic acid;    2-AG;    2-arachidonoyl glycerol;    2-AGE;    2-arachidonyl glyceryl ether;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(02)02341-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The occurrence of the novel proposed endocannabinoid, noladin ether (2-arachidonyl glyceryl ether, 2-AGE) in various rat organs and brain regions, and its inactivation by intact C6 glioma cells, were studied. 2-AGE was measured by isotope dilution liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry, with a detection limit of 100 fmol. A compound with the same mass and chromatographic/chemical properties as 2-AGE was found in whole brain, with the highest amounts in the thalamus and hippocampus. Synthetic [³H]2-AGE was inactivated by intact rat C6 glioma cells by a time- and temperature-dependent process consisting of cellular uptake and partial incorporation into phospholipids. Further data suggested that 2-AGE is taken up by cells via the anandamide/2-arachidonoyl glycerol (2-AG) membrane transporter(s), and biosynthesized in a different way as compared to 2-AG.

【 授权许可】

Unknown