Distinct regions of C‐terminus of the high affinity neurotensin receptor mediate the functional coupling with pertussis toxin sensitive and insensitive G‐proteins

FEBS Letters

Gailly, Philippe¹  Maloteaux, Jean-Marie²  Hermans, Emmanuel²  Najimi, Mustapha² 

[1] Laboratoire de Physiologie Générale des Muscles (FYMU), Département de Physiologie et de Pharmacologie, Université catholique de Louvain 54.10, Avenue Hippocrate 54, B-1200 Brussels, Belgium;Laboratoire de Pharmacologie Expérimentale (FARL), Département de Physiologie et de Pharmacologie, Université catholique de Louvain 54.10, Avenue Hippocrate 54, B-1200 Brussels, Belgium

关键词: Internalization;    Arachidonic acid;    Truncation;    Guanosine-5′-O-(3-thio)triphosphate;    Phospholipase;    NT;    neurotensin;    NTS1;    high affinity neurotensin receptor;    InsP;    inositol phosphate;    CHO;    Chinese hamster ovary;    Gpp(NH)p;    guanylylimidodiphosphate;    GTPγS;    guanosine-5′-O-(3-thio)triphosphate;    PTx;    pertussis toxin;    CHO-NTRwt;    transfected CHO cells expressing the rat NTS1;    NTRΔ372;    truncated NTS1 lacking the last 52 amino acids;    NTRΔ401;    truncated NTS1 lacking the last 23 amino acids;   

DOI  :  10.1016/S0014-5793(02)02285-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The functional coupling of C-terminally truncated mutants of the high affinity rat neurotensin (NT) receptor (NTS1) was characterized in transfected Chinese hamster ovary cells. On cells expressing NTRΔ372 (truncated NTS1 lacking the entire 52 amino acid C-terminus), NT failed to promote [³⁵S]guanosine 5′-[γ-³⁵S]triphosphate binding whereas a robust pertussis toxin (PTx) sensitive response was observed in cells expressing a partially truncated receptor (NTRΔ401 lacking the last 23 residues). Similar results were obtained when measuring the ability of NT to induce the production of arachidonic acid. Since neither deletions impaired the NT-induced phosphoinositide hydrolysis, these results indicate that the membrane proximal region of the C-terminus is specifically involved in the functional coupling of the receptor with PTx sensitive G-proteins. This region was also found to be involved in the control of receptor internalization. However, PTx failed to impair internalization, indicating that these two properties are not directly related.

【 授权许可】

Unknown