A quantity control mechanism regulating levels of the HapE subunit of the Hap complex in Aspergillus nidulans: no accumulation of HapE in hapC deletion mutants

FEBS Letters

Kato, M.¹  Tanaka, A.¹  Kobayashi, T.¹  Oonishi, R.¹  Naruse, F.¹  Hayashi, K.¹  Tanoue, S.¹  Tateyama, Y.¹  Tsukagoshi, N.¹ 

[1] Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan

关键词: CCAAT-binding complex;    Hap complex;    Subunit assembly;    Aspergillus nidulans;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(02)02266-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Aspergillus nidulans CCAAT-binding complex (Hap complex) consists of at least three subunits, HapB, HapC and HapE. To investigate the quantity control mechanisms of the subunits during assembly of the Hap complex, reconstitution studies with the recombinant subunits and extracts prepared from the respective hap subunit deletion mutants were carried out. Furthermore, Western blot analysis of the Hap subunits and Northern blot analysis of the hap genes with the respective deletion mutants were also performed. From all the results together, it was suggested that the number of the HapC molecule could adjust that of the HapE molecule by forming stable heterodimers prior to assembly of the Hap complex.

【 授权许可】

Unknown