A compact view of isochores in the draft human genome sequence

FEBS Letters

Pačes, Jan¹  Bernardi, Giorgio³  Clay, Oliver²  Pavlı́ček, Adam³ 

[1] Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo 2, Prague CZ-16637, Czech Republic;Laboratory of Molecular Evolution, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy;Laboratoire de Génétique Moléculaire, Institut Jacques Monod, 2 place Jussieu, 75005 Paris, France

关键词: Genome organization;    Mammalian DNA;    Analytical ultracentrifugation;    Compositional homogeneity;    GC;    molar ratio of G+C in DNA;   

DOI  :  10.1016/S0014-5793(01)03283-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prior to genome sequencing, information on base composition (GC level) and its variation in mammalian genomes could be obtained using density gradient ultracentrifugation. Analyses using this approach led to the conclusion that mammalian genomes are organized into mosaics of fairly homogeneous regions, called isochores. We present an initial compositional overview of the chromosomes of the recently available draft human genome sequence, in the form of color-coded moving window plots and corresponding GC level histograms. Results obtained from the draft human genome sequence agree well with those obtained or deduced earlier from CsCl experiments. The draft sequence now permits the visualization of the mosaic organization of the human genome at the DNA sequence level.

【 授权许可】

Unknown