DNA intercalation in neutral multilamellar membranes

FEBS Letters

Pott, Tanja¹  Roux, Didier¹ 

[1]Centre de Recherche Paul Pascal – CNRS, Av. A. Schweitzer, 33600 Pessac, France

关键词: DNA;    Encapsulation;    Multilamellar vesicle;    Phospholipid;    Diacylglycerophosphatidylcholine;    X-ray diffraction;    CL;    cationic lipids;    NL;    neutral lipids;    ρ;    lipid-to-DNA weight ratio;    ρ*;    NL-to-encapsulated DNA weight ratio;    SAXS;    small angle X-ray scattering;    sMLV;    small multilamellar vesicles;    sMLVDNA;    DNA-containing small multilamellar vesicles;   

DOI  :  10.1016/S0014-5793(01)03315-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a small angle X-ray scattering study of the DNA/neutral lipid/water system showing that it is possible to confine DNA into a neutral multilamellar phase at high lipid-to-DNA weight ratio, despite the lack of electrostatic interactions in this system. This phase is characterized by a 2D ordering of the DNA molecules intercalated between the neutral bilayers of a 3D smectic phase as shown from the presence of a DNA–DNA correlation peak and the 1D electron density profile of the multilamellar phase. We further demonstrate that it is possible to disperse this phase as small multilamellar vesicles encapsulating high amounts of DNA.

【 授权许可】

Unknown