Carbonyl formation on a copper‐bound prion protein fragment, PrP23–98, associated with its dopamine oxidase activity

FEBS Letters

Shiraishi, Noriyuki¹  Nishikimi, Morimitsu¹ 

[1]Department of Biochemistry, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, Japan

关键词: Prion protein;    Copper;    Carbonyl;    Dopamine;    L-Ascorbate;    PrP;    prion protein;    PrP23–98;    recombinant human PrP residues 23–98;    DTPA;    diethylenetriamine-N;    N;    N′;    N′′;    N′′-pentaacetic acid;    BCS;    bathocuproinedisulfonic acid;   

DOI  :  10.1016/S0014-5793(01)03324-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino-terminal part of prion protein (PrP), containing a series of octapeptide repeats with the consensus sequence PHGGGWGQ, has been implicated in the binding of copper ion. This region possesses amino acid residues susceptible to oxidation, such as histidine, lysine, arginine and proline. In this study, we have investigated copper-catalyzed oxidation of an N-terminal part of human PrP, PrP_23–98, that was prepared by the recombinant DNA technique. Carbonyl formations on copper-bound PrP_23–98 induced by dopamine and L-ascorbate were analyzed kinetically, and it was found that the redox cycling of PrP_23–98-bound copper, especially induced by dopamine, was coupled to the formation of carbonyls on the protein.

【 授权许可】

Unknown