Potent transforming activity of the small GTP‐binding protein Rit in NIH 3T3 cells: evidence for a role of a p38γ‐dependent signaling pathway

FEBS Letters

Gutkind, J.Silvio¹  Teramoto, Hidemi¹  Behbahani, Babak¹  Miyazaki, Hiroshi¹  Sakabe, Kaoru¹  Chikumi, Hiroki¹  Zohar, Muriel¹ 

[1] Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 9000 Rockville Pike, Building 30, Room 212, Bethesda, MD 20892-4330, USA

关键词: Rit;    Mitogen-activated protein kinase;    p38γ;    Focus formation;   

DOI  :  10.1016/S0014-5793(01)03264-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel branch of the Ras family, Rit, was recently identified. Rit exhibits a distinct C-terminus and effector domain, and does not activate mitogen-activated protein kinase (MAPK) but can cooperate with Raf to transform fibroblasts. Here, we found that when overexpressed, activated mutants of Rit transform NIH 3T3 cells efficiently, and stimulate p38γ but not MAPK, p38α, p38β, p38δ, or ERK5. Furthermore, we provide evidence that p38γ activation is required for the ability of Rit to stimulate gene expression and cellular transformation. These findings suggest that this unique GTPase stimulates proliferative pathways distinct from those regulated by other Ras family members.

【 授权许可】

Unknown