Expression and functional role of bTRPC1 channels in native endothelial cells

FEBS Letters

Lovisolo, Davide¹  Fiorio Pla, Alessandra¹  Munaron, Luca¹  Antoniotti, Susanna¹ 

[1] Department of Animal and Human Biology, University of Torino, Via Accademia Albertina 13, I-10123 Torino, Italy

关键词: Transient receptor potential-C1;    Basic fibroblast growth factor;    Calcium influx;    Endothelial cells;   

DOI  :  10.1016/S0014-5793(01)03256-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpc1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells.

【 授权许可】

Unknown