FEBS Letters | |
Expression and functional role of bTRPC1 channels in native endothelial cells | |
Lovisolo, Davide1  Fiorio Pla, Alessandra1  Munaron, Luca1  Antoniotti, Susanna1  | |
[1] Department of Animal and Human Biology, University of Torino, Via Accademia Albertina 13, I-10123 Torino, Italy | |
关键词: Transient receptor potential-C1; Basic fibroblast growth factor; Calcium influx; Endothelial cells; | |
DOI : 10.1016/S0014-5793(01)03256-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpc1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells.
【 授权许可】
Unknown
【 预 览 】
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