Glucosamine modulates IL‐1‐induced activation of rat chondrocytes at a receptor level, and by inhibiting the NF‐κB pathway

FEBS Letters

Bécuwe, P.¹  Bianchi, A.¹  Terlain, B.²  Netter, P.²  Dauça, M.¹  Magdalou, J.²  Bordji, K.²  Gouze, J.N.² 

[1] Laboratoire de Biologie Cellulaire du Développement, Unité Propre de Recherche de l'Enseignement Supérieur 2402, Faculté des Sciences, Université Henri Poincaré, 54500 Vandoeuvre-lès-Nancy, France;UMR 7561 CNRS-Université Henri Poincaré-Nancy I, Physiopathologie et Pharmacologie Articulaires, Faculté de Médecine, P.O. Box 184, 54505 Vandoeuvre-lès-Nancy, France

关键词: Glucosamine;    Rat chondrocyte;    Osteoarthritis;    Nuclear factor κB;    Interleukin-1β;    OA;    osteoarthritis;    PG;    proteoglycan;    IL-1;    interleukin-1;    IL-1RII;    type II IL-1β receptor;    NF-κB;    nuclear factor-κB;    AP-1;    activator protein-1;    IL-1ra;    IL-1 receptor antagonist;    GlcAT-I;    UDP-glucuronosyltransferase I;    ROS;    reactive oxygen species;    FCS;    fetal calf serum;    RT-PCR;    reverse transcriptase polymerase chain reaction;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(01)03255-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently reported that glucosamine reversed the decrease in proteoglycan synthesis and in UDP-glucuronosyltransferase I mRNA expression induced by interleukin-1β (IL-1β) [Arthritis Rheum. 44 (2001) 351–360]. In the present work, we show that glucosamine does not exert the same effects when chondrocytes were stimulated with reactive oxygen species (ROS). In order to better understand its mechanism of action, we determined if glucosamine could prevent the binding of IL-1β to its cellular receptors or could interfere with its signaling pathway at a post-receptor level. Addition of glucosamine to rat chondrocytes treated with IL-1β or with ROS decreased the activation of the nuclear factor κB, but not the activator protein-1. After treatment with IL-1β, glucosamine increased the expression of mRNA encoding the type II IL-1β receptor. These results emphasize the potential role of two regulating proteins of the IL-1β signaling pathway that could account for the beneficial effect of glucosamine in osteoarthritis.

【 授权许可】

Unknown