PSI‐O, a new 10‐kDa subunit of eukaryotic photosystem I

FEBS Letters

Haldrup, Anna¹  Scheller, Henrik Vibe¹  Jensen, Poul Erik¹  Knoetzel, Jürgen¹  Mant, Alexandra¹ 

[1] Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark

关键词: Arabidopsis;    Chloroplast import;    Photosynthesis;    Photosystem I;    psaO;    Photosystem I-O;    Chl;    chlorophyll;    LHC;    light-harvesting complex;    Lhca;    light-harvesting chlorophyll a/b-protein of photosystem I;    Lhcb;    light-harvesting chlorophyll a/b-protein of photosystem II;    PS;    photosystem;    WT;    wild type;   

DOI  :  10.1016/S0014-5793(01)03253-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel polypeptide with an apparent molecular mass of 9 kDa was detected after sodium dodecyl sulphate–polyacrylamide gel electrophoresis of Arabidopsis photosystem I (PSI) and was N-terminally sequenced. Corresponding cDNA clones encode a precursor protein of 140 amino acid residues which was imported into isolated intact chloroplasts and processed to the mature protein, designated PSI-O. The mature protein has two transmembrane helices and a calculated mass of 10 104 Da. The PSI-O protein was also shown to be present in PSI isolated from barley and spinach, and was essentially absent in chloroplast grana. Expressed sequences encoding similar proteins are available from many species of plants and green algae.

【 授权许可】

Unknown