Flow cytometry of isolated mitochondria during development and under some pathological conditions

FEBS Letters

Medina, José M²  López-Mediavilla, Casilda²  Orfao, Alberto¹ 

[1]Departamento de Medicina, Unidad de Citometria, Centro de Investigaciones del Cancer, Universidad de Salamanca, Salamanca, Spain

[2]Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain

关键词: Mitochondrion;    Flow cytometry;    RCR;    respiratory control ratio;    Rh-123;    rhodamine-123;    ROS;    reactive oxygen species;    PTPC;    permeability transition pore complex;   

DOI  :  10.1016/S0014-5793(01)03260-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondria play an essential role in the generation of the energy needed for eukaryotic cell life and in the release of molecules involved in initiation of cell death. Here we review the changes in isolated mitochondrial fluorescent populations as distinguished by flow cytometry during postnatal development and their regulation by thyroid hormones and catecholamines. The use of flow cytometry in the study of mitochondrial changes occurring under hypothyroidism, alcohol abuse and aging is also reviewed.

【 授权许可】

Unknown