NGF‐dependent and tissue‐specific transcription of vgf is regulated by a CREB–p300 and bHLH factor interaction

FEBS Letters

Levi, Andrea²  Molfetta, Rosa¹  Nasi, Sergio¹  Mandolesi, Georgia¹  Mosca, Laura¹  Illi, Barbara¹  Soucek, Laura¹  Gargano, Silvia¹  Jucker, Richard¹  Pennuto, Maria¹ 

[1]Centro Acidi Nucleici CNR, Dipartimento Genetica e Biologia Molecolare, Università La Sapienza, P. le A. Moro 5, 00185 Rome, Italy

[2]Istituto di Neurobiologia CNR, 00156 Rome, Italy

关键词: Neurotrophin;    Vgf;    Transcription;    Basic helix–loop–helix;    cAMP response element binding protein;    p300;    bHLH;    basic helix–loop–helix;    NGF;    nerve growth factor;    BDNF;    brain-derived neurotrophic factor;    NT3–6;    neurotrophins 3–6;    CRE;    cAMP response element;    CREB;    CRE binding protein;   

DOI  :  10.1016/S0014-5793(01)03227-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein complexes bound to this region. One complex, containing HEB (ubiquitous basic helix–loop–helix (bHLH)), bound the promoter in non-neuronal cells and was involved in repressing vgf expression. Neurotrophin-dependent transcription was mediated by the second complex, specific for neuronal cells, which included CRE binding protein and MASH1 (neuro-specific bHLH), bound the CCAAT motif, and was target of neurotrophin signalling. The interaction, mediated by p300, of different transcription factors may add specificity to the neurotrophin response.

【 授权许可】

Unknown