Introns in protein‐coding genes in Archaea

FEBS Letters

Watanabe, Yoh-ichi¹  Kawarabayasi, Yutaka³  Yamagishi, Akihiko²  Kikuchi, Hisasi³  Oshima, Tairo²  Inaba, Toshiro¹  Kita, Kiyoshi¹  Yokobori, Shin-ichi² 

[1] Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;Department of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 1092-0392, Japan;Biotechnology Center, National Institute of Technology and Evaluation, 2-49-10 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan

关键词: Bulge–helix–bulge motif;    Pre-mRNA splicing;    Cbf5p;    Aeropyrum pernix;    Sulfolobus solfataricus;    Sulfolobus tokodaii;    PCR;    polymerase chain reaction;    ORF;    open reading frame;    CBF5;    centromere-binding factor 5;   

DOI  :  10.1016/S0014-5793(01)03219-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Introns in protein-coding genes are ubiquitous in eukaryotic cells, but pre-mRNA splicing has yet to be reported in archaeal and its viral genomes. We present evidence of introns in genes encoding a homolog of eukaryotic Cbf5p (centromere-binding factor 5; a subunit of a small nucleolar ribonucleoprotein) in three Archaea; Aeropyrum pernix, Sulfolobus solfataricus and Sulfolobus tokodaii. Splicing of pre-mRNAs in vivo was demonstrated by reverse transcriptase-mediated polymerase chain reaction. The exon–intron boundaries of these genes are predicted to be folded into a structure similar to the bulge–helix–bulge motif, suggesting that splicing of these pre-mRNAs probably depends on the splicing system elucidated for archaeal pre-tRNAs and rRNAs.

【 授权许可】

Unknown