F‐actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self‐organized reaction–diffusion wave

FEBS Letters

Vicker, Michael G¹ 

[1] Department of Biology-Chemistry, University of Bremen, Leobener Str./NW2, D-28359 Bremen, Germany

关键词: Cell locomotion;    Oscillation;    Self-organization;    Autocatalytic wave;    Filamentous actin;    Dictyostelium discoideum;    GFP;    green fluorescent protein;    F-actin;    filamentous actin;   

DOI  :  10.1016/S0014-5793(01)03207-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dynamics may also yield insights into locomotory mechanisms. Confocal observations of live Dictyostelium cells, expressing a green fluorescent protein–actin fusion protein, demonstrate oscillating supramolecular patterns of filamentous actin throughout the cell, which generate pseudopodia at the cell edge. The distinctively dissipative spatio-temporal behavior of these structures provides strong evidence that reversible actin filament assembly propagates as a self-organized, chemical reaction–diffusion wave.

【 授权许可】

Unknown