Sarcolemma phospholipid structure investigated by immunogold electron microscopy and 31P NMR spectroscopy with lanthanide ions

FEBS Letters

Bondon, Arnaud²  Cavalier, Annie³  Le Rumeur, Elisabeth⁴  Moreau, Céline⁴  Segalen, Jacqueline⁴  Le Floch, Marie¹  Traı̈kia, Mounir⁴  Leray, Geneviève⁴  Rocher, Chantal⁴  Thomas, Daniel³ 

[1] Verres et Céramiques, UMR CNRS 6512, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France;Laboratoire de Chimie Organométallique et Biologique, UMR CNRS 6509, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France;Canaux et récepteurs membranaires, UMR CNRS 6026, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France;Laboratoire de RMN en Biologie et Médecine (LRMBM-UPRES 2230), Faculté de Médecine, CS 34317, 35043 Rennes Cedex, France

关键词: Phospholipid trans-bilayer distribution;    Lanthanide ion;    Magic angle spinning nuclear magnetic resonance;    Vesicle sidedness;    MAS;    magic angle spinning;    CSA;    chemical shift anisotropy;    PL;    phospholipid;   

DOI  :  10.1016/S0014-5793(01)03199-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The biological functions of plasma membranes depend greatly on the biophysical properties resulting from protein and phospholipid structure. We investigated the phospholipid structure of the normal sarcolemma membrane, which is known to be highly dysfunctional in myopathies. Combining electron microscopy and ³¹P nuclear magnetic resonance (NMR) spectroscopy on isolated sarcolemma vesicles, we find that (i) the sarcolemma vesicles maintain the in-vivo cellular sidedness, (ii) the phospholipid mobility is close to that observed in model membranes (similar lateral diffusion coefficients and spin–lattice T ₁ relaxation times). Using broad-band and magic angle spinning ³¹P NMR spectroscopy with lanthanide ions (Pr³⁺), it is possible to quantify the distribution of phospholipids between internal and external membrane layers, showing that the trans-bilayer distribution is highly asymmetrical.

【 授权许可】

Unknown