CLK‐1/Coq7p is a DMQ mono‐oxygenase and a new member of the di‐iron carboxylate protein family

FEBS Letters

Rea, Shane¹ 

[1] SapIENCE, Montreal, QC, Canada H2X 1X8

关键词: Aging;    Alternative oxidase;    Senescence;    Gerontogene;    Bacterioferritin;    Clk-1;    AO;    alternative oxidase;    DMQ;    2-polyprenyl-3-methyl-6-methoxy-1;    4-benzoquinone;    DMQ2;    2-diprenyl-3-methyl-6-methoxy-1;    4-benzoquinone;    FR;    fumarate reductase;    HBB;    3-hexaprenyl-4-hydroxybenzoate;    Q;    coenzyme Q or ubiquinone;    ROS;    reactive oxygen species;    RQ;    rhodoquinone;    TRC;    tandem repeat in CLK-1;   

DOI  :  10.1016/S0014-5793(01)03099-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Strains of Caenorhabditis elegans mutant for clk-1 exhibit a 20–40% increase in mean lifespan. clk-1 encodes a mitochondrial protein thought to be either an enzyme or regulatory molecule acting within the ubiquinone biosynthesis pathway. Here CLK-1 is shown to be related to the ubiquinol oxidase, alternative oxidase, and belong to the functionally diverse di-iron-carboxylate protein family which includes bacterioferritin and methane mono-oxygenase. Construction and analysis of a homology model indicates CLK-1 is a 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone mono-oxygenase as originally predicted. Analysis of known CLK-1/Coq7p mutations also supports this notion. These findings raise the possibility of developing CLK-1-specific inhibitors to test for lifespan extension in higher organisms.

【 授权许可】

Unknown