Phased A‐tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

FEBS Letters

Matsushita, Osamu¹  Tamai, Eiji¹  Miyata, Shigeru¹  Okabe, Akinobu¹  Katayama, Seiichi² 

[1] Department of Microbiology, Faculty of Medicine, Kagawa Medical University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan;Department of Biochemistry and Chemistry, Faculty of Science, Okayama University of Science, 1-1, Ridai-cho, Okayama 700-0005, Japan

关键词: Curved DNA;    α Subunit;    RNA polymerase;    Gene expression;    Phospholipase C;    Clostridium perfringens;    PCR;    polymerase chain reaction;    RNAP;    RNA polymerase;    RNAPα;    α subunit of RNA polymerase;    αCTD;    C-terminal domain of α subunit;    αNTD;    N-terminal domain of α subunit;   

DOI  :  10.1016/S0014-5793(01)03148-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

【 授权许可】

Unknown