Histamine increases the expression of LOX‐1 via H2 receptor in human monocytic THP‐1 cells

FEBS Letters

Kimura, Satoshi¹  Murata, Yoshitaka²  Sasaguri, Yasuyuki¹  Xu, Hui¹  Arima, Nobuyuki¹  Hamada, Tetsuo¹  Nomaguchi, Masako²  Tanimoto, Akihide¹ 

[1] Department of Pathology and Cell Biology, School of Medicine, University of Occupational Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu 807-8555, Japan;Department of Kyurin Omtest Laboratory, Kyurin Corporation, Japan

关键词: Monocyte/macrophage;    Histamine receptor;    Lectin-like oxidized low-density lipoprotein receptor-1;    Cyclic AMP response element;    Atherosclerosis;    CRE;    cyclic AMP response element;    CREB;    CRE binding protein;    Bt2-cAMP;    dibutyryl cyclic AMP;    FCS;    fetal calf serum;    HDC;    histidine decarboxylase;    LOX-1;    lectin-like oxidized low-density lipoprotein receptor-1;    LDL;    low-density lipoprotein;    NBT;    nitroblue tetrazolium;    PG;    prostaglandin;    TPA;    tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/S0014-5793(01)03073-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a member of the scavenger receptor family, and is known to be expressed in monocytes/macrophages. We investigated the effect of histamine on the expression of LOX-1 in cells of the human monocytic leukemia cell line THP-1. Histamine as well as forskolin and dibutyryl cyclic AMP (Bt₂-cAMP) stimulated the THP-1 monocytes to express the LOX-1 gene at the transcription level. This histamine effect on LOX-1 gene expression, via the histamine H2 receptor-mediated cAMP signal transduction pathway, was reduced after differentiation of the cells into macrophages, even though forskolin and Bt₂-cAMP still enhanced the gene expression. The alteration of the responsiveness of LOX-1 expression to histamine was related to suppressed expression of the H2 receptor in THP-1 macrophages. The switch of the predominant class of histamine receptors between H1 and H2 would modulate the effects of histamine on LOX-1 gene expression in monocytes and macrophages, and therefore, would play a certain role in the inflammatory aspects of atherogenesis.

【 授权许可】

Unknown