The role of PDR13 in tolerance to high copper stress in budding yeast

FEBS Letters

Lee, Youngsook¹  Yang, Young-Yell¹  Kim, Do-Young¹  Song, Won-Yong¹ 

[1] Division of Molecular Life Sciences, Pohang University of Science and Technology, Pohang 790-784, South Korea

关键词: PDR13;    High Cu stress;    Budding yeast;    Metallothionein;    MTs;    methallothioneins;    PDR;    pleiotropic drug resistance;    ABC transporter;    ATP-binding cassette transporter;   

DOI  :  10.1016/S0014-5793(01)03038-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PDR13 in Saccharomyces cerevisiae contributes to drug resistance via sequential activation of PDR1 and PDR5. In this study, we found that a PDR13 deletion mutant was hypersensitive to Cu²⁺ compared to the wild-type counterpart. The Cu²⁺ tolerance mechanism mediated by Pdr13 does not seem to involve Pdr1 or Pdr5, since mutants harboring a deletion of either the PDR1 or PDR5 gene did not show elevated Cu²⁺ sensitivity. Instead, we found that the PDR13 null mutant could not express CUP1 or CRS5 metallothionein at wild-type levels when subjected to high Cu²⁺ stress. These results suggest that Pdr13 contributes to high Cu²⁺ tolerance of S. cerevisiae, at least in part, via a mechanism involving metallothionein expression.

【 授权许可】

Unknown