Involvement of CCAAT/enhancer‐binding protein in regulation of the rat serine:pyruvate/alanine:glyoxylate aminotransferase gene expression

FEBS Letters

Kitagawa, Masatoshi²  Oda, Toshiaki²  Hayashi, Hideharu¹  Ichiyama, Arata²  Uchida, Chiharu²  Sugiyama, Tsuyoshi¹ 

[1]Department of Internal Medicine III, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan

[2]First Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan

关键词: Serine:pyruvate aminotransferase;    CCAAT/enhancer-binding protein;    Two transcription start sites;    Downstream promoter;   

DOI  :  10.1016/S0014-5793(01)03010-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted to characterize the downstream promoter responsible for generation of peroxisomal SPT/AGT. The minimal downstream promoter was confined to the +21–+90 region. We demonstrated that C/EBPα and C/EBPβ bound around the downstream start site (+66) contribute to the promoter activity. The downstream promoter activity is also regulated positively by a short inverted repeat, located 20–30 bp upstream of the downstream start site, through a protein factor(s) bound to this region. On the other hand, the sequence just downstream of the start site may negatively regulate the promoter activity.

【 授权许可】

Unknown