Human homolog of mouse tescalcin associates with Na+/H+ exchanger type‐1

FEBS Letters

Mailänder, Jens¹  Dedio, Jürgen¹  Müller-Esterl, Werner¹ 

[1] Institute for Biochemistry II, University of Frankfurt, Theodor-Stern-Kai 7, D-60590 Frankfurt, Germany

关键词: Tescalcin;    Na+/H+ exchanger type-1;    Calcineurin homologous protein;    Yeast two-hybrid system;    CHP;    calcineurin homologous protein;    CNB;    calcineurin B subunit;    DIG;    digoxigenin;    GST;    glutathione S-transferase;    HA;    hemagglutinin;    NHE1;    Na+/H+ exchanger type-1;    PBS;    phosphate-buffered saline;    TSC;    tescalcin;   

DOI  :  10.1016/S0014-5793(01)02986-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel regulatory protein, tescalcin (TSC), recently isolated from mouse embryonic testes, has been implicated in gonadal differentiation. Employing the yeast two-hybrid system with the Na⁺/H⁺ exchanger type-1 (NHE1) carboxyterminal domain as a bait we have identified a novel NHE1-associated protein of 214 amino acid residues representing the human homolog of mouse TSC (96.7% identity). Co-precipitation experiments demonstrated the interaction of human TSC with NHE1 in vitro and in vivo, and ⁴⁵Ca²⁺ overlay assay revealed that TSC binds Ca²⁺. Immunofluorescence studies indicated that TSC is prominent in cellular lamellipodia where it colocalizes with NHE1. Abundant expression of TSC mRNA in the heart suggests that TSC may play important role(s) in concert with NHE1 in cardiac tissues.

【 授权许可】

Unknown