Transcriptional repression of the human p53 gene by cobalt chloride mimicking hypoxia

FEBS Letters

Rho, Hyune Mo¹  Lee, Hyeon¹  Lee, Seok Geun¹ 

[1] School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea

关键词: p53 tumor suppressor gene;    Transcription;    Hypoxia;    Cobalt chloride;   

DOI  :  10.1016/S0014-5793(01)02989-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The switch to an angiogenic phenotype is known to be a fundamental determinant of neoplastic growth and tumor progression. We herein report that the transcription of the human p53 gene was repressed by treatment with a hypoxia-mimicking concentration of cobalt chloride and alone by hypoxia-inducible factor 1α. Analyses of serial deletions, site-directed mutageneses and heterologous promoter systems showed that the site responsible for the repression by both factors was the E-box element in the promoter of the p53 gene. These results alongside previous data suggest that the loss of p53 including the transcriptional repression may play an important role in the angiogenic switch during tumorigenesis.

【 授权许可】

Unknown