Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton

FEBS Letters

Anděra, Ladislav¹  Milgram, Sharon L.²  Brdičková, Naděžda¹  Angelisová, Pavla¹  Hořejšı́, Václav¹  Špička, Jiřı́¹  Brdička, Tomáš¹ 

[1] Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vı́deňská 1083, 14220 Prague 4, Czech Republic;Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

关键词: Raft;    Microdomain;    Cytoskeleton;    Adapter protein;    PDZ domain;    CFTR;    cystic fibrosis transmembrane conductance regulator;    DMEM;    Dulbecco's modified Eagle's medium;    EBP50;    ERM-binding phosphoprotein 50 kDa;    ERM;    ezrin–radixin–moesin;    FCS;    fetal calf serum;    GEM;    glycosphingolipid-enriched membrane microdomain;    PAG;    phosphoprotein associated with GEMs;    PBS;    phosphate-buffered saline;    PTK;    protein tyrosine kinase;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;    Y2H;    yeast 2-hybrid system;   

DOI  :  10.1016/S0014-5793(01)02955-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphoprotein associated with GEMs (PAG), also known as Csk-binding protein (Cbp), is a broadly expressed palmitoylated transmembrane adapter protein found in membrane rafts, also called GEMs (glycosphingolipid-enriched membrane microdomains). PAG is known to bind and activate the essential regulator of Src-family kinases, cytoplasmic protein tyrosine kinase Csk. In the present study we used the yeast 2-hybrid system to search for additional proteins which might bind to PAG. We have identified the abundant cytoplasmic adapter protein EBP50 (ezrin/radixin/moesin (ERM)-binding phosphoprotein of 50 kDa), also known as NHERF (Na⁺/H⁺ exchanger regulatory factor), as a specific PAG-binding partner. The interaction involves the C-terminal sequence (TRL) of PAG and N-terminal PDZ domain(s) of EBP50. As EBP50 is known to interact via its C-terminal domain with the ERM-family proteins, which in turn bind to actin cytoskeleton, the PAG–EBP50 interaction may be important for connecting membrane rafts to the actin cytoskeleton.

【 授权许可】

Unknown