Calf thymus Hsc70 and Hsc40 can substitute for DnaK and DnaJ function in protein renaturation but not in bacteriophage DNA replication

FEBS Letters

Konieczny, Igor¹  Ziemienowicz, Alicja²  Hübscher, Ulrich² 

[1] Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;Department of Veterinary Biochemistry, University of Zürich, Winterthurerstr. 190, CH-8057 Zurich, Switzerland

关键词: Hsc70;    Heat shock protein 40;    DnaK;    DnaJ;    Protein renaturation;    DNA replication;   

DOI  :  10.1016/S0014-5793(01)02923-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calf thymus (ct) Hsc70 has been shown previously to reactivate heat-inactivated prokaryotic and eukaryotic enzymes, while DnaK was able to reactivate solely prokaryotic enzymes. Here, we report on isolation from calf thymus of a DnaJ homolog, ctHsc40, and on testing of its cooperative function in three different assays: (i) reactivation of heat-inactivated DNA polymerases, (ii) stimulation of the ATPase activity of ctHsc70 chaperone, and (iii) replication of bacteriophage λ DNA. Surprisingly, ctHsc70/ctHsc40 chaperones were found to reactivate the denatured prokaryotic and eukaryotic enzymes but not to promote bacteriophage λ DNA replication, suggesting species specificity in DNA replication.

【 授权许可】

Unknown