FEBS Letters | |
Calf thymus Hsc70 and Hsc40 can substitute for DnaK and DnaJ function in protein renaturation but not in bacteriophage DNA replication | |
Konieczny, Igor1  Ziemienowicz, Alicja2  Hübscher, Ulrich2  | |
[1] Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland;Department of Veterinary Biochemistry, University of Zürich, Winterthurerstr. 190, CH-8057 Zurich, Switzerland | |
关键词: Hsc70; Heat shock protein 40; DnaK; DnaJ; Protein renaturation; DNA replication; | |
DOI : 10.1016/S0014-5793(01)02923-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Calf thymus (ct) Hsc70 has been shown previously to reactivate heat-inactivated prokaryotic and eukaryotic enzymes, while DnaK was able to reactivate solely prokaryotic enzymes. Here, we report on isolation from calf thymus of a DnaJ homolog, ctHsc40, and on testing of its cooperative function in three different assays: (i) reactivation of heat-inactivated DNA polymerases, (ii) stimulation of the ATPase activity of ctHsc70 chaperone, and (iii) replication of bacteriophage λ DNA. Surprisingly, ctHsc70/ctHsc40 chaperones were found to reactivate the denatured prokaryotic and eukaryotic enzymes but not to promote bacteriophage λ DNA replication, suggesting species specificity in DNA replication.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020311040ZK.pdf | 297KB | download |