Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae

FEBS Letters

Doignon, François¹  Weinachter, Caroline¹  Larrieu, Isabelle¹  Roumanie, Olivier¹  Crouzet, Marc¹ 

[1] Laboratoire de Biologie Moléculaire et de Séquençage, Institut de Biochimie et Génétique Cellulaires, UMR CNRS 5095, P.O. Box 64, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France

关键词: Rho protein;    GTPase activating protein;    BAG7;    LRG1;    RGD2;    Saccharomyces cerevisiae;    ATA;    3-amino-1;    2;    4-triazole;    DEP domain;    dishevelled-Egl-10-pleckstrin domain;    FCH domain;    Fes/CIP4 homology domain;    LIM domain;    Lin-11-Isl-1-Mec-3 domain;    GAP;    GTPase activating protein;    GST;    glutathione S-transferase;    MESG;    7-methyl-6-thioguanosine;    PCR;    polymerase chain reaction;    Pi;    inorganic phosphate;    RGS;    regulator of G protein signalling;   

DOI  :  10.1016/S0014-5793(01)02906-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and Rho5p. In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac-like GTPase Rho5.

【 授权许可】

Unknown