A novel serum chitinase that is expressed in bovine liver

FEBS Letters

Morimatsu, Masami²  Iwanaga, Toshihiko¹  Suzuki, Masako²  Syuto, Bunei²  Yamashita, Tetsuro³ 

[1] Graduate School of Veterinary Medicine, Hokkaido University, Kita 18-Nishi 9, Kita-ku, Sapporo 060-0818, Japan;Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan;Agricultural Life Science, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan

关键词: Chitin;    Mammalian chitinase;    Bovine serum;    Liver;    Innate immunity;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/S0014-5793(01)02893-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chitinases are ubiquitous chitin-fragmenting hydrolases. They are synthesized by a vast array of organisms, including those not composed of chitin. Here, we describe a novel serum chitinase (chitin-binding protein b04, CBPb04), which is expressed in bovine liver. Although CBPb04 is secreted as an endocrine chitinase, it shows higher homology with human gastrointestinal tract exocrine chitinase (AMCase) than with macrophage endocrine chitinase (human chitotriosidase). This suggests that cows have a specific defense against chitin-containing microorganisms. CBPb04 mRNA is expressed in hepatocytes. This is the first report of a hepatogenic mammalian chitinase.

【 授权许可】

Unknown