Pim‐1 translocates sorting nexin 6/TRAF4‐associated factor 2 from cytoplasm to nucleus

FEBS Letters

Koike, Naoyuki³  Iguchi-Ariga, Sanae M.M.¹  Ariga, Hiroyoshi³  Tamai, Katsuyuki²  Ishibashi, Yuki³  Maita, Hiroshi³  Yano, Minoru² 

[1] College of Medical Technology, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan;Ina Laboratories, MBL Co. Ltd., Ina 396, Japan;Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan

关键词: Tumor necrosis factor receptor-associated factor 4-associated factor 2;    Sorting nexin 6;    Pim-1;    TFAF2;    tumor necrosis factor receptor-associated factor 4-associated factor 2;    SNX6;    sorting nexin 6;    PX;    phox homology;    HP-1;    heterochromatin protein 1;   

DOI  :  10.1016/S0014-5793(01)02881-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pim-1, an oncogene product of serine/threonine kinase, has been found to play roles in apoptosis induction/suppression, cell-cycle progression and transcriptional regulation by phosphorylating the target proteins involved in these processes. The target proteins phosphorylated by Pim-1, including p100, Cdc25A, PAP-1 and heterochromatin protein 1, have been identified. The precise functions of Pim-1, however, are still poorly understood. In this study, we identified tumor necrosis factor receptor-associated factor 4-associated factor 2/sorting nexin 6 (TFAF2/SNX6) as a Pim-1-binding protein, and we found that TFAF2/SNX6 was phosphorylated and translocated from the cytoplasm to nucleus by Pim-1. This translocation of the protein was not affected by Pim-1-dependent phosphorylation. Since sorting nexins, including TFAF2/SNX6, have been reported to be located in the cytoplasm or membrane by association with several receptors of tyrosine- or serine/threonine-kinase, this is the first report of TFAF2/SNX6 being located in the nucleus after binding to Pim-1.

【 授权许可】

Unknown