Apigenin decreases expression of the myofibroblast phenotype

FEBS Letters

Goldstein, Ronald H¹  Rishikof, David C¹  Kuang, Ping-Ping¹  Ricupero, Dennis A¹  Poliks, Christine F¹ 

[1] Pulmonary Center and the Department of Biochemistry, Boston University School of Medicine and the Boston VA Medical Center, Boston, MA 02118, USA

关键词: α1(I) Collagen;    mRNA stability;    Apigenin;    Flavonoid;    α-Smooth muscle actin;    Myofibroblast;    IMR-90;   

DOI  :  10.1016/S0014-5793(01)02737-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effect of the dietary flavonoid apigenin on myofibroblast function. We report that in myofibroblasts treated with apigenin, proliferation and basal levels of α1(I) collagen and α-smooth muscle actin mRNAs were markedly reduced. Apigenin also attenuated the transforming growth factor-β-stimulated increases of α1(I) collagen and α-smooth muscle actin mRNAs. Characterization of the apigenin effects indicates that apigenin reduces both the stability of the α1(I) collagen mRNA and the rate of transcription of the α1(I) collagen gene through a cycloheximide-sensitive pathway. Western blot analyses indicate that Akt activity is reduced in apigenin-treated myofibroblasts.

【 授权许可】

Unknown