Replication of the rat aldolase B locus differs between aldolase B‐expressing and non‐expressing cells

FEBS Letters

Tsutsumi, Ken-ichi²  Tamai, Katsuyuki¹  Saitoh, Yasushi²  Miyagi, Satoru²  Zhao, Yung-peng² 

[1] Ina Laboratories, MBL Co., Ltd., Ina, Nagano 396-0002, Japan;Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Ueda, Morioka, Iwate 020-8550, Japan

关键词: Replication origin;    Origin selection;    Aldolase B gene;    Promoter;    Gene expression;    PCR;    polymerase chain reaction;    BrdU;    bromodeoxyuridine;    AldB;    aldolase B;    HSP;    heat-shock protein;   

DOI  :  10.1016/S0014-5793(01)02849-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously reported a rat chromosomal origin of DNA replication (oriA1) that encompassed the aldolase B (AldB) gene promoter. Here, we examined utilization of oriA1 in AldB-expressing and non-expressing cells. The results suggested the occurrence of mutually exclusive regulation between DNA replication and transcription. Nascent strand abundance as assayed by competitive polymerase chain reaction using bromodeoxyuridine-labeled nascent DNA indicated that oriA1 is not utilized in AldB-expressing cells, while it is fired in non-expressing cells. In the latter non-expressing cells, the replication fork seemed to slow at 20–22 kb downstream of oriA1.

【 授权许可】

Unknown