Polycystin‐1: immunoaffinity isolation and characterisation by mass spectrometry

FEBS Letters

Abuknesha, Ramadan A.¹  Malhas, Ashraf N.¹  Price, Robert G.¹ 

[1] Department of Biochemistry, School of Life Sciences, King's College London, London SE1 9NN, UK

关键词: Autosomal dominant polycystic kidney disease;    Polycystin-1;    Immunoaffinity purification;    Anti-peptide antibody;    Mass spectrometry;    Protein identification;    ADPKD;    autosomal dominant polycystic kidney disease;    MALDI-TOF MS;    matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry;   

DOI  :  10.1016/S0014-5793(01)02842-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polycystin-1 is a putative 460 kDa membrane protein with a unique structure and is possibly representative of a new family of proteins. Its structure suggests an involvement in cell signalling and cell–matrix interactions. The amino acid sequence of polycystin-1 has to date been predicted from its gene sequence. This, to our knowledge, is the first report of the isolation and analysis of polycystin-1 at the protein level using mass spectrometry to confirm its predicted structure. The availability of purified polycystin-1 will allow a new approach to unravelling the complexity of the cell–cell and cell–matrix interactions of this large molecule in normal cells and its perturbation in disease.

【 授权许可】

Unknown