A single‐stranded telomere binding protein in the nematode Caenorhabditis elegans

FEBS Letters

Lee, Junho¹  Yi, Soo Young¹  Joeng, Kyu Sang¹  Cho, Jin Won¹  Kweon, Jai Uk¹  Chung, In Kwon¹ 

[1] Department of Biology, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul 120-749, South Korea

关键词: Single-stranded telomere binding protein;    Gel mobility shift assay;    Core sequence;    Two-dimensional gel electrophoresis;    Southwestern analysis;    Caenorhabditis elegans;   

DOI  :  10.1016/S0014-5793(01)02821-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We identified and characterized a protein (STB-1) from the nuclear extract of Caenorhabditis elegans that specifically binds single-stranded telomere DNA sequences, but not the corresponding RNA sequences. STB-1 binding activity is specific to the nematode telomere, but not to the human or plant telomere. STB-1 requires the core nucleotides of GCTTAGG and three spacer nucleotides in front of them for binding. While any single nucleotide change in the core sequence abolishes binding, the spacer nucleotides tolerate substitution. STB-1 was determined to be a basic protein of 45 kDa by Southwestern analyses. STB-1 forms a stable complex with DNA once bound to the telomere.

【 授权许可】

Unknown