Effects of histamine and interleukin‐4 synthesized in arterial intima on phagocytosis by monocytes/macrophages in relation to atherosclerosis

FEBS Letters

Murata, Yoshitaka³  Higuchi, Satoshi²  Sasaguri, Yasuyuki¹  Xu, Hui¹  Arima, Nobuyuki¹  Hamada, Tetsuo¹  Makishima, Kazumi²  Tanimoto, Akihide¹ 

[1] Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan;Department of Otolaryngology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu 807-8555, Japan;Department of Kyurin Omtest Laboratory, Kyurin Corporation, Yahatanishi-ku, Kitakyushu 806-0046, Japan

关键词: Histamine;    Interleukin-4;    Histidine decarboxylase;    LOX-1;    Atherosclerosis;    U937 cell;    Jurkat cell;    Promoter assay;   

DOI  :  10.1016/S0014-5793(01)02823-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the localization of histidine decarboxylase (HDC), which is the rate-limiting enzyme that generates histamine from histidine, in human aorta/coronary artery. RT-PCR and immunohistochemical staining revealed that the HDC gene was expressed in monocytes/macrophages and T cells in the arterial intima but not in smooth muscle cells in either the arterial intima or the media. A luciferase promoter assay with U937 and Jurkat cells demonstrated that interleukin-4 (IL-4) inhibited the expression of the HDC gene. In contrast, among a scavenger receptor family, IL-4 as well as histamine up-regulated U937 cells to express the LOX-1 gene but not the SR-A gene, which genes encode receptors that scavenge oxidized lipids. These findings suggest that histamine synthesized in the arterial wall participates in the initiation and progression of atherosclerosis and that IL-4 can act as an important inhibitory and/or stimulatory factor in the function of monocytes/macrophages modulated by histamine in relation to the process of atherosclerosis.

【 授权许可】

Unknown