Xenopus kidney hyaluronidase‐1 (XKH1), a novel type of membrane‐bound hyaluronidase solely degrades hyaluronan at neutral pH1

FEBS Letters

Lepperdinger, Günter¹  Müllegger, Johannes¹  Reitinger, Stephan¹ 

[1] Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstr. 11, A-5020 Salzburg, Austria

关键词: Hyaluronan;    Hyaluronidase;    PH-20;    Kidney;   

DOI  :  10.1016/S0014-5793(01)02813-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In search for Xenopus laevis hyaluronidase genes, a cDNA encoding a putative PH-20-like enzyme was isolated. In the adult frog, this mRNA was only found to be expressed in the kidney and therefore named XKH1. When expressed by means of cRNA injection into frog oocytes, XKH1 solely exhibited at physiologic ionic strength hyaluronidase activity at neutral pH and in weakly acidic solutions. The enzyme was inactive below pH 5.4. In addition to hyaluronic acid hydrolysis, chondroitin sulfate also was degraded at low yield as assessed by fluorophore-assisted carbohydrate electrophoresis analysis of the degradation products. The enzyme is sorted to the outer surface of the cell membrane of XKH1 expressing oocytes. From there, it could not be removed by phospholipase C nor was secreted hyaluronidase activity detectable. We conclude that XKH1 represents a membrane-bound hyaluronan-degrading enzyme exclusively expressed in cells of the adult frog kidney where it either may be involved in the reorganization of the extracellular architecture or in supporting physiological demands for proper renal functions.

【 授权许可】

Unknown