D1′ centers are less efficient than normal photosystem II centers

FEBS Letters

Funk, Christiane²  Wiklund, Ronney²  Schröder, Wolfgang P.³  Jansson, Christer¹ 

[1] Department of Plant Biology, The Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden;Department of Biochemistry and Biophysics, The Arrhenius Laboratories, Stockholm University, SE-106 91 Stockholm, Sweden;Section for Natural Sciences, Södertörn University College, P.O. Box 4101, SE-141 04 Huddinge, Sweden

关键词: D1;    D1′;    Photosystem II;    psbA;    Synechocystis;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    HEPES;    N-(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid;    QA;    primary plastoquinone electron acceptor in photosystem II;    QB;    secondary plastoquinone electron acceptor in photosystem II;   

DOI  :  10.1016/S0014-5793(01)02794-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

One prominent difference between the photosystem II (PSII) reaction center protein D1′ in Synechocystis 6803 and normal D1 is the replacement of Phe-186 in D1 with leucine in D1′. Mutants of Synechocystis 6803 producing only D1′, or containing engineered D1 proteins with Phe-186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash-induced oxygen evolution. Compared to D1-containing PSII centers, D1′ centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash-induced O₂ evolution pattern was unaffected. In the F186 mutants, both the P680⁺/Q_A ⁻ recombination and O₂ oscillation pattern were noticeably perturbed.

【 授权许可】

Unknown