Sea urchin sperm cation‐selective channels directly modulated by cAMP

FEBS Letters

Darszon, Alberto²  Sánchez, Daniel²  Labarca, Pedro¹ 

[1] Centro de Estudios Cientı́ficos, Valdivia, Chile;Departamento de Genética y Fisiologı́a Molecular, Instituto de Biotecnologı́a, UNAM, Apdo. 510-3, Cuernavaca, Morelos, 62250, Mexico

关键词: Speract;    cAMP;    Hyperpolarization-activated cyclic nucleotide-gated channel;    Sperm;    Sea urchin;    AR;    acrosome reaction;    ASW;    artificial sea water;    8Br-cAMP;    8-bromoadenosine 3′:5′-cyclic monophosphate;    8Br-cGMP;    8-bromoguanosine 3′:5′-cyclic monophosphate;    cAMP;    cyclic adenosine monophosphate;    cGMP;    cyclic guanosine monophosphate;    db-cAMP;    N 2;    2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate;    V m;    transmembrane potential;    V c;    command potential;    V r;    resting membrane potential;    V rev;    reversal potential;    HCN;    hyperpolarization-activated cyclic nucleotide-gated;    H7;    1-(5-isoquinolinylsulfonyl)-2-methylpiperazine;    IBMX;    3-isobutyl-1-methylxanthine;    pHi;    intracellular pH;    SPIH;    hyperpolarization- and nucleotide-activated channel from sea urchin sperm;   

DOI  :  10.1016/S0014-5793(01)02713-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Components of the sea urchin outer egg jelly layer such as speract drastically change second messenger levels and membrane permeability in sperm. Ion channels are deeply involved in the sperm–egg dialogue in sea urchin and other species. Yet, due to the small size of sperm, studies of ion channels and their modulation by second messengers in sperm are scarce. In this report we offer the first direct evidence that cation-selective channels upwardly regulated by cAMP operate in sea urchin sperm. Due to their poor selectivity among monovalent cations, channel activation in seawater could contribute to sperm membrane repolarization during the speract response.

【 授权许可】

Unknown