A sensitive filter retention assay for the detection of PrPSc and the screening of anti‐prion compounds

FEBS Letters

Hartl, F.Ulrich¹  Winklhofer, Konstanze F¹  Tatzelt, Jörg¹ 

[1] Department of Cellular Biochemistry, Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

关键词: Anti-prion compound;    Bovine spongiform encephalopathy;    Creutzfeldt–Jakob disease;    Prion;   

DOI  :  10.1016/S0014-5793(01)02692-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A hallmark of prion diseases is the accumulation of an abnormally folded prion protein, denoted PrP^Sc. Here we describe a new and highly sensitive method for the detection of PrP^Sc in brain and other tissue samples that utilizes both PrP^Sc diagnostic criteria in combination; protease resistance and aggregation. Upon filtration of tissue extracts derived from scrapie- or bovine spongiform encephalopathy-infected animals, PrP^Sc is retained and detected on the membranes. Laborious steps such as SDS–PAGE and Western blotting are avoided with concomitant gain in sensitivity and reliability. The new procedure also proved useful in a screen for anti-prion compounds in a scrapie-infected cell culture model.

【 授权许可】

Unknown