Mice lacking histidine decarboxylase exhibit abnormal mast cells

FEBS Letters

Falus, András⁴  Gertsenstein, Marina⁸  Ishigaki-Suzuki, Satsuki²  Ohuchi, Kazuo¹  Ohtsu, Hiroshi²  Hara, Masahiro³  Hori, Yoshio²  Buzás, Edit⁴  Kovács, Péter⁴  Okada, Mikiko³  Tanaka, Satoshi⁶  Numayama-Tsuruta, Keiko²  Terui, Tadashi³  Hellman, Lars⁷  Csaba, György⁴  Hirasawa, Noriyasu¹  Watanabe, Takehiko²  Pejler, Gunnar⁷  Ichikawa, Atsushi⁶  Makabe-Kobayashi, Yoko²  Mar, Lynn⁸  Nagy, András⁸  Sakurai, Eiko²  Kittel, Ágnes⁵  Tchougounova, Elena⁷ 

[1] Department of Pathophysiological Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan;Department of Cellular Pharmacology, Tohoku University School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai 980-8575, Japan;Department of Dermatology, Tohoku University School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai 980-8575, Japan;Department of Genetics, Cell- and Immunobiology, Semmelweis Medical University, 4 Nagyvárad tér, H-1089 Budapest, Hungary;Institute of Experimental Medicine, Budapest, Hungary;Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan;Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, BMC, P.O. Box 575, 751 23 Uppsala, Sweden;Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada M5G 1X5

关键词: Mast cell;    Rodent;    Knockout;    Histamine;    HDC;    histidine decarboxylase;    α-FMH;    α-fluoromethyl histidine;    BMMC;    bone marrow-derived mast cell;    MMCP;    mouse mast cell protease;    MC-CPA;    mast cell carboxypeptidase A;   

DOI  :  10.1016/S0014-5793(01)02663-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Histidine decarboxylase (HDC) synthesizes histamine from histidine in mammals. To evaluate the role of histamine, we generated HDC-deficient mice using a gene targeting method. The mice showed a histamine deficiency and lacked histamine-synthesizing activity from histidine. These HDC-deficient mice are viable and fertile but exhibit a decrease in the numbers of mast cells while the remaining mast cells show an altered morphology and reduced granular content. The amounts of mast cell granular proteases were tremendously reduced. The HDC-deficient mice provide a unique and promising model for studying the role of histamine in a broad range of normal and disease processes.

【 授权许可】

Unknown