Malaria parasite Plasmodium gallinaceum up‐regulates host red blood cell channels

FEBS Letters

Staines, Henry M.¹  Ellory, J.Clive¹  Egée, Stéphane²  Thomas, Serge L.Y.²  Lapaix, Franck²  Kaestner, Lars² 

[1] University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK;Centre National de la Recherche Scientifique, UPR 9042, Station Biologique, Place G. Teissier, P.O. Box 74, 29682 Roscoff Cedex, France

关键词: Erythrocyte;    Malaria;    Ionic channel;    Permeation pathway;    Plasmodium gallinaceum;   

DOI  :  10.1016/S0014-5793(01)02579-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The properties of the malaria parasite-induced permeability pathways in the host red blood cell have been a major area of interest particularly in the context of whether the pathways are host- or parasite-derived. In the present study, the whole-cell configuration of the patch-clamp technique has been used to show that, compared with normal cells, chicken red blood cells infected by Plasmodium gallinaceum exhibited a 5–40-fold larger membrane conductance, which could be further increased up to 100-fold by raising intracellular Ca²⁺ levels. The increased conductance was not due to pathways with novel electrophysiological properties. Rather, the parasite increased the activity of endogenous 24 pS stretch-activated non-selective cationic (NSC) and 62 pS calcium-activated NSC channels, and, in some cases, of endogenous 255 pS anionic channels.

【 授权许可】

Unknown