Non‐invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green fluorescent protein (GFP)

FEBS Letters

Hayashi, Jun-Ichi²  Kaneda, Hideki¹  Sato, Akitsugu¹  Shitara, Hiroshi¹  Taya, Choji¹  Iwasaki, Kuniko¹  Yonekawa, Hiromichi¹ 

[1] Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan;Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

关键词: Mitochondrion;    Mitochondrial DNA transmission;    Green fluorescent protein;    Transgenic mouse;    Embryogenesis;   

DOI  :  10.1016/S0014-5793(01)02574-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using high sensitive polymerase chain reaction (PCR), we previously demonstrated that selective elimination of sperm mitochondrial DNA occurred during early embryogenesis in mouse. To analyze the process morphologically in more detail, a non-invasive, real-time observation of sperm mitochondria was used. Transgenic mice that express green fluorescent protein (GFP) exclusively in mitochondria (mtGFP-tg mice) were generated. The fluorescence in mtGFP-tg mice was strong and stable enough to carry out repeated observations under confocal laser scanning microscopy. In these mtGFP-tg mice it was revealed that the sperm mitochondria were selectively eliminated from egg cytoplasm during the two-cell stage of early embryogenesis. Therefore, mtGFP-tg mice should contribute to studies on sequential or repeated analysis of mitochondria.

【 授权许可】

Unknown