Genes modulated by histone acetylation as new effectors of butyrate activity

FEBS Letters

Della Pietra, Valentina¹  Oliva, Adriana¹  Indaco, Stefania¹  Criniti, Vittoria¹  Borriello, Adriana¹  Yamamoto, Tadashi²  Della Ragione, Fulvio¹  Zappia, Vincenzo¹ 

[1] Department of Biochemistry and Biophysics ‘F. Cedrangolo’, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy;Department of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan

关键词: Butyrate;    Trichostatin A;    Histone hyperacetylation;    Histone deacetylase;    GATA-2;    tob-1;    BuA;    butyrate;    TSA;    trichostatin A;    HDAC;    histone deacetylase;   

DOI  :  10.1016/S0014-5793(01)02539-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A wealth of evidence correlates the chemopreventive activity of a fiber-rich diet with the production of butyrate. In order to identify the genes transcriptionally modulated by the molecule, we analyzed the expression profile of butyrate-treated colon cancer cells by means of cDNA expression arrays. Moreover, the effect of trichostatin A, a specific histone deacetylase inhibitor, was studied. A superimposable group of 23 genes out of 588 investigated is modulated by both butyrate and trichostatin A. Among them, a major target was tob-1, a gene involved in the control of cell cycle. tob-1 is also up-regulated by butyrate in a neuroblastoma-derived cell line, and its overexpression in the colon cells caused growth arrest. Our findings represent an extensive analysis of genes modulated by butyrate and identify completely new effectors of its biological activities.

【 授权许可】

Unknown