Model of interaction of the IL‐1 receptor accessory protein IL‐1RAcP with the IL‐1β/IL‐1RI complex

FEBS Letters

Boraschi, Diana²  Bossù, Paola⁵  Casadio, Rita³  Martin, Michael U¹  Neumann, Detlef¹  Tagliabue, Aldo⁴  Frigimelica, Elisabetta³ 

[1] Pharmakologie, Medizinische Hochschule Hannover, D-30623 Hannover, Germany;Laboratory of Immunobiology, IMD, National Research Council, Via G. Moruzzi 1, I-56124 Pisa, Italy;Laboratory of Biocomputing, University of Bologna, Via S. Giacomo 9/2, Bologna, Italy;ALTA Srl, Via delle Vigne 12, I-53035 Monteriggioni, Italy;Dompé SpA, Via Campo di Pile, I-67100 L'Aquila, Italy

关键词: Interleukin 1;    Receptor chain;    Accessory protein;    Molecular modeling;    IL-1;    interleukin 1;    IL-1R;    IL-1 receptor;    IL-1RAcP;    IL-1R accessory protein;    Ig;    immunoglobulin;    LAF;    lymphocyte-activating factor;   

DOI  :  10.1016/S0014-5793(01)02515-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A preliminary model has been calculated for the activating interaction of the interleukin 1 receptor (IL-1R) accessory protein IL-1RAcP with the ligand/receptor complex IL-1β/IL-1R_I. First, IL-1RAcP was modeled on the crystal structure of IL-1R_I bound to IL-1β. Then, the IL-1RAcP model was docked using specific programs to the crystal structure of the IL-1β/IL-1R_I complex. Two types of models were predicted, with comparable probability. Experimental data obtained with the use of IL-1β peptides and antibodies, and with mutated IL-1β proteins, support the BACK model, in which IL-1RAcP establishes contacts with the back of IL-1R_I wrapped around IL-1β.

【 授权许可】

Unknown