Identification of novel mast cell genes by serial analysis of gene expression in cord blood‐derived mast cells

FEBS Letters

Watanabe, Takehiko¹  Nakajima, Toshiharu²  Kubota, Yasuhiko¹  Kuramasu, Atsuo¹  Matsumoto, Kenji²  Sun, Xu-Ming¹  Ohtsu, Hiroshi¹  Saito, Hirohisa² 

[1] Department of Cellular Pharmacology, Tohoku University School of Medicine, 2-1, Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan;Department of Allergy and Immunology, National Children's Medical Research Center, 3-35-31, Taishido, Setagaya-ku, Tokyo 154-8509, Japan

关键词: Mast cell;    Serial analysis of gene expression;    Human;    Transcript;    SAGE;    serial analysis of gene expression;    MC(s);    mast cell(s);    CB;    cord blood;    Btk;    Bruton's tyrosine kinase;    HDC;    histidine decarboxylase;    EST;    expressed sequence tag;    PBMNC(s);    peripheral blood mononuclear cell(s);    PG;    prostaglandin;    MITF;    mi locus-encoded transcription factor;    LT;    leukotriene;   

DOI  :  10.1016/S0014-5793(01)02477-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene expression profile of human cord blood-derived mast cells (MCs) was investigated using serial analysis of gene expression (SAGE). A total of 22 914 tags, representing 9181 unique transcripts, were sequenced. By selecting tags that were detected more frequently in MCs than in other tissues, genes characteristic of MCs were enriched. Reverse transcription-PCR and the high-density oligonucleotide array hybridization confirmed the validity of our SAGE result. About 70% of the selected genes were previously uncharacterized. Northern blot analysis showed the MC-specific expression of selected genes. This inventory will be useful to identify novel genes with important functions in MCs.

【 授权许可】

Unknown