The Drosophila proteins Pelle and Tube induce JNK/AP‐1 activity in mammalian cells

FEBS Letters

Schmitz, M.Lienhard¹  Bacher, Susanne¹  Dröge, Wulf¹  Großhans, Jörg² 

[1] German Cancer Research Center (DKFZ), Department of Immunochemistry (G0200), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany;Max-Planck Institut für Entwicklungsbiologie, Spemannstrasse 35/III, D-72076 Tübingen, Germany

关键词: Interleukin-1 signaling;    Pelle;    Tube;    c-Jun N-terminal kinase;    AP-1;    Nuclear factor-κB;    IL-1;    interleukin-1;    IL-1R;    IL-1 receptor;    MAPK;    mitogen-activated protein kinase;    JNK;    c-Jun N-terminal kinase;    IL-1AcP;    IL-1 accessory protein;    DD;    death domain;    IRAK;    IL-1 receptor-associated kinase;    MAP3K;    MAPK kinase kinase;    DJNK;    Drosophila JNK protein;    DTRAF-1;    Drosophila TRAF-1;   

DOI  :  10.1016/S0014-5793(01)02454-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mammalian interleukin-1 (IL-1) signal transduction pathways display remarkable homology to the Toll signaling cascade in Drosophila. To address the question whether members of the Drosophila Toll pathway are functional in mammalian cells, inactive and constitutively active versions of the protein kinase Pelle and its regulator Tube were expressed in HeLa cells and tested for their impact on IL-1-dependent signaling events. The Drosophila proteins failed to induce the IL-1-responsive transcription factor, nuclear factor-κB, but selectively activated the IL-1-regulated kinase, c-Jun N-terminal kinase (JNK), thus resulting in elevated AP-1 activity. Activation of JNK/AP-1 activity was seen upon expression of a Pelle mutant lacking its C-terminal half or by a membrane-bound and multimerised Tube protein, showing the functionality of the Drosophila proteins in mammalian cells.

【 授权许可】

Unknown