A small peptide derived from Flt‐1 (VEGFR‐1) functions as an angiogenic inhibitor

FEBS Letters

Jois, Seetharama D.S.¹  Tan, Darren C.W.²  Lim, Derrick K.F.²  Ge, Ruowen²  Xin, Lixia²  Kini, R.Manjunatha² 

[1] Department of Pharmacy, National University of Singapore, Singapore 119260, Singapore;Department of Biological Sciences, National University of Singapore, Singapore 119260, Singapore

关键词: Vascular endothelial growth factor (vascular permeability factor);    Flt-1(VEGFR-1);    Angiogenesis;    Miles’ assay;    Chick chorioallantoic membrane;    VEGF;    vascular endothelial growth factor;    VPF;    vascular permeability factor;    HUVEC;    human umbilical cord vascular endothelial cells;    CAM;    chick chorioallantoic membrane;    CD;    circular dichroism;    NMR;    nuclear magnetic resonance;   

DOI  :  10.1016/S0014-5793(01)02314-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vascular endothelial growth factor (VEGF) is an angiogenic stimulator which functions through two endothelial specific tyrosine kinase receptors, Flt-1 and Flk-1. In this work, we show that an 11-amino acid peptide derived from the second immunoglobulin-like domain of Flt-1 functions as an angiogenic inhibitor in chick chorioallantoic membrane and inhibited VEGF-induced vascular permeability in Miles’ assay without binding to VEGF directly. Circular dichroism and nuclear magnetic resonance analyses indicate that this peptide forms a stable extended structure in solution, presumably β-sheet structure and is most likely existing as a dimer. Our results suggest that this small peptide functions as an angiogenic inhibitor by inhibiting VEGF function through a non-VEGF binding mechanism.

【 授权许可】

Unknown