Non‐destructive quantitation of spermine in human prostate tissue samples using HRMAS 1H NMR spectroscopy at 9.4 T

FEBS Letters

Wu, Chin-lee³  Cheng, Leo Ling³  Gonzalez, R.Gilberto¹  Smith, Matthew R.² 

[1] Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA;Department of Hematology and Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA;Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA

关键词: Human prostate;    Spermine;    Proton nuclear magnetic resonance spectroscopy;    High resolution magic angle spinning;    Intact tissue specimen;    Citrate;    NMR;    nuclear magnetic resonance;    HRMAS;    high resolution magic angle spinning;   

DOI  :  10.1016/S0014-5793(01)02329-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present the results of a study of human prostate specimens evaluated by high resolution magic angle spinning ¹H nuclear magnetic resonance (NMR) spectroscopy at 400 MHz (9.4 T) and by quantitative histopathology. We demonstrate that NMR and pathology data can be obtained from the same intact specimens, and report for the first time a linear correlation between the NMR measured concentration of spermine, a proposed endogenous inhibitor to prostate cancer growth, and the volume percentage of normal prostatic epithelial cells as quantified by histopathology. Our results show that NMR may serve as a critical tool for the investigation of the inhibitory mechanism of spermine in human subjects.

【 授权许可】

Unknown