Mutations in the fatty acid elongation 1 gene are associated with a loss of β‐ketoacyl‐CoA synthase activity in low erucic acid rapeseed

FEBS Letters

Puyaubert, J²  Lessire, R²  Renard, M¹  Delseny, M³  Roscoe, T.J³ 

[1] UMR INRA-ENSAR, Amélioration des Plantes et Biotechnologies Végétales, B.P. 35327, 35653 Le Rheu, France;Laboratoire de Biogenèse Membranaire, CNRS UMR 5544, Université V. Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France;Laboratoire de Génome et Développement des Plantes, CNRS UMR 5096, Université de Perpignan, 52 Avenue de Villeneuve, 66860 Perpignan Cedex, France

关键词: Acyl-CoA elongase;    β-Ketoacyl-CoA synthase;    Erucic acid;    Fatty acid elongation 1;    Brassica napus;   

DOI  :  10.1016/S0014-5793(01)02243-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low erucic acid rapeseed (LEAR) is characterised by a near absence of very long chain fatty acids in the seed oil which has been correlated with a lack of acyl-CoA elongation activity. Here we show that the absence of acyl-CoA and ATP-dependent elongation activities in microsomes isolated from LEAR embryos is associated with an absence of β-ketoacyl-CoA synthase activity encoded by the Bn-fatty acid elongation 1 (FAE1) genes. Size exclusion chromatography of solubilised microsomes revealed the presence of a high molecular mass acyl-CoA elongase complex in high erucic acid rapeseed which was absent in microsomes isolated from LEAR seeds. Although transcripts for the Bn-FAE1 genes were detected in LEAR embryos, immunoblots using antisera raised against the β-ketoacyl-CoA synthase indicated an absence of this protein. Comparison of the deduced amino acid sequences of immature embryo cDNAs reveals that LEAR alleles of Bn-FAE1 encode variant β-ketoacyl-CoA synthase proteins.

【 授权许可】

Unknown