Expression and characterization of a magnetosome‐associated protein, TPR‐containing MAM22, in Escherichia coli

FEBS Letters

Fukumori, Yoshihiro¹  Okuda, Yoshihiro² 

[1] Department of Biology, Faculty of Science, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;Graduate School of Teikyo University of Science and Technology, Kitatsuru-gun, Yamanashi 409-0193, Japan

关键词: TPR motif;    Magnetosome;    Magnetospirillum magnetotacticum;    MAM22;    magnetosome-specific 22 kDa protein from Magnetospirillum magnetotacticum;    PAGE;    polyacrylamide gel electrophoresis;    TPR;    tetratrico-peptide repeat;    PCR;    polymerase chain reaction;    IPTG;    isopropyl β-D-thiogalactopyranoside;   

DOI  :  10.1016/S0014-5793(01)02178-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A magnetosome-associated protein, MAM22, contains a TPR domain (five TPR motifs and one putative TPR motif) that has been known to mediate protein–protein interactions. We expressed the mam22 gene in Escherichia coli and found that the purified MAM22 was reversibly self-aggregated by NaCl. The structural model of MAM22 which has been proposed on the basis of the crystal structure of the N-terminal TPR domain of a human Ser/Thr protein phosphatase suggests the novel hydrophobic colloidal features of MAM22 with TPR motifs.

【 授权许可】

Unknown