Different susceptibility to oxidation of proline and arginine residues of apolipoprotein B‐100 among subspecies of low density lipoproteins

FEBS Letters

Pietzsch, Jens¹  Julius, Ulrich¹ 

[1] Institute and Policlinic of Clinical Metabolic Research, Medical Faculty ‘Carl Gustav Carus’, Technical University, Fetscherstrasse 74, D-01307 Dresden, Germany

关键词: Low density lipoprotein;    Apolipoprotein B-100;    Protein oxidation;    Atherogenesis;    apoB-100;    apolipoprotein B-100;    HAVA;    5-hydroxy-2-aminovaleric acid;    LDL;    low density lipoprotein;   

DOI  :  10.1016/S0014-5793(01)02181-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

γ-Glutamyl semialdehyde is a primary oxidation product of apolipoprotein (apo) B-100 proline (Pro) and arginine (Arg) side chain residues. By reduction γ-glutamyl semialdehyde forms 5-hydroxy-2-aminovaleric acid (HAVA). Here we describe the application of sensitive and specific HAVA measurement to characterize the formation of γ-glutamyl semialdehyde in several domains of apoB-100 in LDL₁ (S_f 7–12) and LDL₂ (S_f 0–7) subfractions subjected to oxidative damage in the presence of iron in vitro. Results suggest that susceptibility of apoB-100 Pro and Arg residues toward oxygen radicals drastically changes along the lipoprotein metabolic cascade.

【 授权许可】

Unknown